Enzymes and Inhibitors
New England Biolabs, Inc. EcoRV-HF – 20000 units
A high fidelity restriction endonuclease that recognizes the sequence GAT_^ATC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. HaeIII – 15000 units
A restriction endonuclease that recognizes the sequence GG_^CC.
New England Biolabs, Inc. SspI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. NruI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence TCG^CGA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. AvrII – 100 units
A restriction endonuclease that recognizes the sequence C^CTAG_G.
New England Biolabs, Inc. DpnI – 1000 units
A restriction endonuclease that recognizes the sequence GA_^TC.
- DpnI cleaves only when its recognition site is methylated
- Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation
- Methylation-sensitive restriction enzyme
- Time-Saver qualified for digestion in 5-15 minutes
- 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer)
New England Biolabs, Inc. NsiI – 1000 units
A restriction endonuclease that recognizes the sequence A_TGCA^T.
Sorbent Technologies Inc Silica for DCC, w/UV254, 60A, 63-200um, 500g
Silica for Dry Column Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying industrial quantities of compounds. DCC links analytical thin layer chromatography (TLC) with preparative column chromatography (CC). Like TLC, DCC is a "non-elution" method utilizing Nylon Foil Tubing as a support. In TLC solvent flow is by capillary action going against gravity whereas in DCC, solvent flow is by gravity. Like Column Chromatography, columns of most any size may be filled in DCC to separate or clean up very large quantities of materials. Silica Gel for DCC consists of a UV254 fluorescent indicator and a silica gel with a porosity of 60A, particle distribution of 63-200um, and a quantity of 500g.
New England Biolabs, Inc. ScaI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. HincII – 1000 units
A restriction endonuclease that recognizes the sequence GTY_^RAC.
New England Biolabs, Inc. NaeI – 500 units
A restriction endonuclease that recognizes the sequence GCC_^GGC.
New England Biolabs, Inc. BmtI-HF – 300 units
A high fidelity restriction endonuclease that recognizes the sequence G_CTAG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.